Affiliation:
1. Departments of Microbiology, Michigan State University, East Lansing, Michigan 48823
2. Departments of Microbiology, The University of Michigan, Ann Arbor, Michigan 48104
Abstract
Homogeneous fragments of exosporium were extricated in centigram amounts from dormant spores of
Bacillus cereus
and analyzed for intrinsic constituents. The membrane proved to be chemically complex but not unique, consisting mainly of protein (52%), amino and neutral polysaccharides (20%), lipids (18%), and ash (4%). Seventeen common amino acids were identified by chromatography, and were present in usual proportions except for low levels of cystine-cysteine, methionine, tyrosine, and histidine. Glucosamine was the only amino sugar, and glucose and rhamnose were the principal neutral sugars. The lipid fraction contained 5.5% cardiolipin as the only phospholipid, 12.5% neutral lipids, and at least 19 fatty acids, among which normal C
16
and C
18
ones predominated. Calcium and phosphorus occurred in the ash. Small amounts of teichoic, ribonucleic, and dipicolinic acids were believed to represent contamination.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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