Affiliation:
1. Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan
2. Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854
Abstract
ABSTRACT
The developmentally regulated gene
dofA
, identified from pulse-labeling experiments by two-dimensional gel electrophoresis, and its homologue,
dofB
, were cloned and characterized in
Myxococcus xanthus
. Deletion of
dofA
and
dofB
did not affect the vegetative growth and development of
M. xanthus. dofA
was specifically expressed during development, while
dofB
expression was observed during vegetative growth and development. The
dofA-lacZ
fusion was introduced into a
fruA
mutant and A, B, C, D, and E extracellular signal mutants. The pattern of
dofA
expression in the C signal mutant was similar to that of the wild-type strain, while
dofA
expression was not detected in the
fruA
mutant. These results are consistent with those of the pulse-labeling experiments.
dofA
expression was reduced in A and E signal mutants, whereas
dofA
expression was delayed in B and D signal mutants. The patterns of expression of the
dofA
gene in the
fruA
mutant and the five signal mutants are strikingly similar to that of the
tps
gene, which encodes protein S, a major component of the outer surface of the myxospore; this result suggests that the
dofA
and
tps
genes are similarly regulated. The involvement of a highly GC-rich inverted repeat sequence (underlined),
CGGCCCCCGA
TTCG
TCGGGGGCCG
, in developmentally regulated
dofA
expression is suggested.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
10 articles.
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