Isolation of Glucocardiolipins from Geobacillus stearothermophilus NRS 2004/3a

Author:

Schäffer Christina1,Beckedorf Anke I.23,Scheberl Andrea1,Zayni Sonja1,Peter-Katalinić Jasna2,Messner Paul1

Affiliation:

1. Zentrum für Ultrastrukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur Wien, A-1180 Vienna, Austria

2. Institut für Medizinische Physik und Biophysik, Universität Münster, D-48149 Münster, Germany

3. Institute for Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912-2620

Abstract

ABSTRACT Glucose-substituted cardiolipins account for about 4 mol% of total phospholipid extracted from exponentially grown cells of Geobacillus stearothermophilus NRS 2004/3a. Individual glucocardiolipin species exhibited differences in fatty acid substitution, with iso-C 15:0 and anteiso-C 17:0 prevailing. The compounds were purified to homogeneity by a novel protocol and precharacterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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