Antimalarial Drug Resistance Profiling of Plasmodium falciparum Infections in Ghana Using Molecular Inversion Probes and Next-Generation Sequencing-Reference-Cited by-同舟云学术

Antimalarial Drug Resistance Profiling of Plasmodium falciparum Infections in Ghana Using Molecular Inversion Probes and Next-Generation Sequencing

Published:2020-03-24 Issue:4 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Mensah Benedicta A.¹²,Aydemir Ozkan³,Myers-Hansen James L.¹,Opoku Millicent¹,Hathaway Nicholas J.³,Marsh Patrick W.³,Anto Francis²,Bailey Jeffrey³⁴,Abuaku Benjamin¹,Ghansah Anita¹

Affiliation:

1. Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra, Ghana

2. School of Public Health, University of Ghana, Accra, Ghana

3. Program in Bioinformatics and Integrative Biology, University of Massachusetts, Worcester, Massachusetts, USA

4. Division of Transfusion Medicine, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA

Abstract

A key drawback to monitoring the emergence and spread of antimalarial drug resistance in sub-Saharan Africa is early detection and containment. Next-generation sequencing methods offer the resolution, sensitivity, and scale required to fill this gap by surveilling for molecular markers of drug resistance. We performed targeted sequencing using molecular inversion probes to interrogate five Plasmodium falciparum genes ( pfcrt , pfmdr1 , pfdhps , pfdhfr , and pfk13 ) implicated in chloroquine, sulfadoxine-pyrimethamine (SP), and artemisinin resistance in two sites in Ghana.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01423-19

Reference84 articles.

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