Improved Direct Viable Count Procedure for Quantitative Estimation of Bacterial Viability in Freshwater Environments-Reference-Cited by-同舟云学术

Improved Direct Viable Count Procedure for Quantitative Estimation of Bacterial Viability in Freshwater Environments

Published:2000-12 Issue:12 Volume:66 Page:5544-5548
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Yokomaku Daisaku¹,Yamaguchi Nobuyasu¹,Nasu Masao¹

Affiliation:

1. Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan

Abstract

ABSTRACT A direct viable count (DVC) procedure was developed which clearly and easily discriminates the viability of bacterial cells. In this quantitative DVC (qDVC) procedure, viable cells are selectively lysed by spheroplast formation caused by incubation with antibiotics and glycine. This glycine effect leads to swollen cells with a very loose cell wall. The viable cells then are lysed easily by a single freeze-thaw treatment. The number of viable cells was obtained by subtracting the number of remaining cells after the qDVC procedure from the total cell number before the qDVC incubation. This improved procedure should provide useful information about the metabolic potential of natural bacterial communities.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.12.5544-5548.2000

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