lac Up-promoter mutants with increased homology to the consensus promoter sequence-Reference-Cited by-同舟云学术

lac Up-promoter mutants with increased homology to the consensus promoter sequence

Published:1985-12 Issue:3 Volume:164 Page:1353-1355
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Mandecki W,Goldman R A,Powell B S,Caruthers M H

Abstract

Four lac promoter mutants were constructed. The mutations increased the homology between the lac promoter and the consensus promoter sequences by introducing the consensus -10 and -35 regions and the consensus spacing of 17 residues between these two regions. The promoter mutants were cloned into a pBR322-derivatized vector upstream from the lacZ gene, and levels of beta-galactosidase were an indication of promoter activity. All mutants exhibited higher activity than did the wild-type promoter.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.164.3.1353-1355.1985

Reference20 articles.

1. In vivo expression of lac promoter variants with altered -10, -35, and spacer sequences;Ackerson J. W.;Cold Spring Harbor Symp. Quant. Biol.,1982

2. The tac promoter: a functional hybrid derived from the trp and lac promoters;deBoer H. A.;Proc. Natl. Acad. Sci. USA,1983

3. Chemical synthesis and biochemical reactivity of bacteriophage lambda Pr promoter;deHaseth P. L.;Nucleic Acids Res.,1983

4. Compilation and analysis of Escherichia coli promoter DNA sequences;Hawley D. K.;Nucleic Acids Res.,1983

5. A new class of promoter mutants in the lactose operon of Escherichia coli;Hopkins J. D.;J. Mol. Biol.,1974

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