Evaluation of a Modular Multiplex-PCR Methicillin-Resistant Staphylococcus aureus Detection Assay Adapted for mecC Detection-Reference-Cited by-同舟云学术

Evaluation of a Modular Multiplex-PCR Methicillin-Resistant Staphylococcus aureus Detection Assay Adapted for mecC Detection

Published:2013-06 Issue:6 Volume:51 Page:1917-1919
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Becker Karsten¹,Larsen Anders R.²,Skov Robert L.²,Paterson Gavin K.³,Holmes Mark A.³,Sabat Artur J.⁴,Friedrich Alexander W.⁴,Köck Robin⁵,Peters Georg¹,Kriegeskorte André¹

Affiliation:

1. Institute of Medical Microbiology, University Hospital Münster, Münster, Germany

2. Statens Serum Institute, Copenhagen, Denmark

3. Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

4. Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

5. Institute of Hygiene, University Hospital Münster, Münster, Germany

Abstract

ABSTRACT A mecC ( mecA LGA251 )-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa -typed Staphylococcus aureus collection comprising 51 mecC -positive MRSA, 240 mecA -positive MRSA, and 50 mecA - and mecC -negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00075-13

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