Identification of Lotus Rhizobia by Direct DNA Hybridization of Crushed Root Nodules-Reference-Cited by-同舟云学术

Identification of Lotus Rhizobia by Direct DNA Hybridization of Crushed Root Nodules

Published:1987-07 Issue:7 Volume:53 Page:1705-1707
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Cooper J. E.¹,Bjourson A. J.¹,Thompson J. K.¹

Affiliation:

1. Agricultural and Food Bacteriology Research Division, Department of Agriculture for Northern Ireland, Belfast BT9 5PX, Northern Ireland

Abstract

Hybridization of crushed Lotus pedunculatus root nodules with 32 P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. ( Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did not occur. Results were comparable to those from colony hybridization.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.53.7.1705-1707.1987

Reference8 articles.

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3. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene;Grunstein M.;Proc. Natl. Acad. Sci. USA,1975

4. DNA colony hybridization to identify Rhizobium strains;Hodgson A. L. M.;J. Gen. Microbiol.,1983

5. Maniatis T. E. F. Fritsch and J. Sambrook. 1982. Molecular NOTES 1707

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