Transformation Frequency of a mariner-Based Transposon in Rickettsia rickettsii-Reference-Cited by-同舟云学术

Transformation Frequency of a mariner-Based Transposon in Rickettsia rickettsii

Published:2011-09-15 Issue:18 Volume:193 Page:4993-4995
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Clark Tina R.¹,Lackey Amanda M.²,Kleba Betsy¹,Driskell Lonnie O.²,Lutter Erika I.¹,Martens Craig³,Wood David O.²,Hackstadt Ted¹

Affiliation:

1. Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840

2. Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, Alabama 36688

3. Genomics Unit, Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840

Abstract

ABSTRACT Transformation frequencies of a mariner -based transposon system in Rickettsia rickettsii were determined using a plaque assay system for enumeration and isolation of mutants. Sequence analysis of insertion sites in both R. rickettsii and R. prowazekii indicated that insertions were random. Transposon mutagenesis provides a useful tool for rickettsial research.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.05279-11

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