Multiplex PCR for Enterotoxigenic, Attaching and Effacing, and Shiga Toxin-Producing Escherichia coli Strains from Calves-Reference-Cited by-同舟云学术

Multiplex PCR for Enterotoxigenic, Attaching and Effacing, and Shiga Toxin-Producing Escherichia coli Strains from Calves

Published:1998-06 Issue:6 Volume:36 Page:1795-1797
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Franck Sophia M.¹,Bosworth Brad T.²,Moon Harley W.¹

Affiliation:

1. Department of Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011,1 and

2. Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 500102

Abstract

ABSTRACT A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.6.1795-1797.1998

Reference28 articles.

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