Affiliation:
1. Department of Bacteriology and Food Research Institute, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706
Abstract
No correlation existed between numbers of organisms and nuclease activity in laboratory-grown cultures of
Staphylococcus aureus
. Nuclease production was inhibited by anaerobic incubation and stimulated by aeration. Strains of
S. aureus
varied in the production of nuclease. The optimum pH for enzyme production was 8.3 and employment of a tris(hydroxymethyl)aminomethane buffer system resulted in increased production of the enzyme as compared with a phosphate buffer. The nuclease was extremely heat-stable and had a D value of 16.6 min at 130 C.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference8 articles.
1. Enzymatic detection of the growth of Staphylococcus aureus in foods;Chesbro W. R.;Appl. Microbiol.,1967
2. A deoxyribonuclease of Micrococcus pyogenes;Cunningham L.;J. Amer. Chem. Soc.,1956
3. Turbidimetric assay of staphylococcal nuclease;Erickson A.;Appl. Microbiol.,1973
4. Frazier W. C. 1967. Determination of heat resistance p. 89-93. In Food microbiology 2nd ed. McGraw-Hill Book Co. Inc. New York.
5. Rapid method for determining the activity of microorganisms on nucleic acids;Jeffries C. D.;J. Bacteriol.,1957
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献