Affiliation:
1. Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, Consejo Nacional de Investigaciones Cientificas y Técnicas, Universidad Nacional de General San Martín, Buenos Aires, Argentina
Abstract
ABSTRACT
The animal pathogen
Brucella abortus
contains a gene
cgt
, which complemented
Sinorhizobium meliloti
nodule development (
ndvA
) and
Agrobacterium tumefaciens
chromosomal virulence (
chvA
) mutants. Complemented strains recovered the presence of anionic cyclic β-1,2-glucan, motility, tumor induction in
A. tumefaciens
, and nodule occupancy in
S. meliloti
, all traits strictly associated with the presence of cyclic β-1,2-glucan in the periplasm. Nucleotide sequencing revealed that
B. abortus cgt
contains a 1,797-bp open reading frame coding for a predicted membrane protein of 599 amino acids (65.9 kDa) that is 58.5 and 59.9% identical to
S. meliloti
NdvA and
A. tumefaciens
ChvA, respectively. Additionally,
B. abortus cgt
, like
S. meliloti ndvA
and
A. tumefaciens chvA
possesses ATP-binding motifs and the ABC signature domain features of a typical ABC transporter. Characterization of Cgt was carried out by the construction of null mutants in
B. abortus
2308 and S19 backgrounds. Both mutants do not transport cyclic β-1,2-glucan to the periplasm, as shown by the absence of anionic cyclic glucan, and they display reduced virulence in mice and defective intracellular multiplication in HeLa cells. These results suggest that cyclic β-1,2-glucan must be transported into the periplasmatic space to exert its action as a virulence factor.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
41 articles.
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