Purification and characterization of two extracellular alkaline phosphatases from a psychrophilic arthrobacter isolate-Reference-Cited by-同舟云学术

Purification and characterization of two extracellular alkaline phosphatases from a psychrophilic arthrobacter isolate

Published:1997-07 Issue:7 Volume:63 Page:2928-2931
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

De Prada P,Brenchley J E

Abstract

Two extracellular, heat-labile alkaline phosphatases were purified from a psychrophilic Arthrobacter isolate, D10. The enzymes were active over different pH ranges, used distinct substrates, and had different kinetic properties. Each enzyme reacted specifically to its own antibody during immunoblot analysis. One had both monophosphatase and diesterase activities.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.63.7.2928-2931.1997

Reference18 articles.

1. A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay;Baykov A. A.;Anal. Biochem.,1988

2. Psychrophilic microorganisms and their cold-active enzymes;Brenchley J. E.;J. Ind. Microbiol. Biotechnol.,1996

3. Production of two extracellular alkaline phosphatases by a psychrophilic Arthrobacter strain;de Prada P.;Appl. Environ. Microbiol.,1996

4. Characterization of a periplasmic 3~:5~-cyclic nucleotide phosphodiesterase gene, cpdP, from the marine symbiotic bacterium Vibrio fischeri;Dunlap P. V.;J. Bacteriol.,1993

5. Growth of the marine Iuminous bacterium Vibrio fischeri on 3~:5~-cyclic AMP: correlation with a periplasmic 3~:5~-cyclic AMP phosphodiesterase;Dunlap P. V.;J. Gen. Microbiol.,1992

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