Effect of Molybdenum Starvation and Tungsten on the Synthesis of Nitrogenase Components in Klebsiella pneumoniae

Author:

Brill Winston J.1,Steiner Ann L.1,Shah Vinod K.1

Affiliation:

1. Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706

Abstract

Klebsiella pneumoniae M5a1 grows well in the presence or absence of molybdenum in media containing excess NH 4 + . However, growth on N 2 is completely dependent on the presence of molybdenum in the medium. Tungstate competes with the molybdate requirement during growth on N 2 . In molybdenum-depleted medium, neither protein component of nitrogenase is active and neither component can be detected antigenically. These data provide evidence that molybdenum is an inducer of nitrogenase synthesis.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference17 articles.

1. Molybdenum in relation to pasture improvement in South Australia;Anderson A. J.;J. Council. Sci. Indust. Res.,1946

2. Tungsten incorporation into Azotobacter vinelandii nitrogenase;Benemann J. R.;FEBS Lett.,1973

3. Molybdan als Katalysator bei der biologischen Stichstoffbindung;Bortels H.;Arch. Mikrobiol.,1930

4. The nitrogenase system from Azotobacter: two-enzyme requirements for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis;Bulen W. A.;Proc. Nat. Acad. Sci. U.S.A.,1966

5. Isolation by crystallization of the Mo-Fe protein of Azotobacter nitrogenase;Burns R. C.;Biochem. Biophys. Res. Commun.,1970

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