Electron Microscopy of the Cell Wall of Rickettsia prowazeki

Author:

Palmer E. L.1,Mallavia L. P.1,Tzianabos T.1,Obijeski J. F.1

Affiliation:

1. Center for Disease Control, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

Abstract

Purified Rickettsia prowazeki were found to undergo morphological changes resembling plasmolysis when stained with uranyl acetate, resulting in rod-like forms. Sequential electron micrographs of disintegrating organisms provide evidence for the cell wall origin of these rod-like forms. The substructure of the cell wall was discerned by using negative-contrast electron microscopy. The wall was found to be composed of repetitive subunits with a periodicity of 13 nm and was surrounded by a thin membrane.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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