Ribonucleic Acid Regulation in Permeabilized Cells of Escherichia coli Capable of Ribonucleic Acid and Protein Synthesis

Author:

Atherly Alan G.1

Affiliation:

1. Department of Genetics, 5 Curtiss Hall, Iowa State University, Ames, Iowa 50010

Abstract

A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference10 articles.

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2. Transcription and translation mechanisms in "permeabilized;Ben-Hanida F.;Biochemie,1971

3. Effects of toluene on Escherichia coli;Jackson R. W.;J. Bacteriol.,1965

4. Titration of gene sites on DNA by DNA-RNA hybridization;Kennell D.;J. Mol. Biol.,1968

5. Regulation of ribosomal RNA synthesis in cold-shocked E. coli;Lazzarini R. A.;Nature N. Biol.,1973

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