Lysis of Bacterial Cell Walls by an Enzyme Isolated from a Myxobacter

Author:

Ensign J. C.1,Wolfe R. S.1

Affiliation:

1. Department of Microbiology, University of Illinois, Urbana, Illinois

Abstract

Ensign , J. C. (University of Illinois, Urbana), and R. S. Wolfe . Lysis of bacterial cell walls by an enzyme isolated from a myxobacter. J. Bacteriol. 90: 395–402. 1965.—An exoenzyme which lyses intact cells, heat-killed cells, and cell walls of Arthrobacter crystallopoietes was purified 60-fold from the growth liquor obtained from a myxobacter (strain AL-1). The lytic enzyme was produced during growth of the organism in a number of complex media, the maximal amount of enzyme being produced in yeast extract broth. The purified enzyme lysed at different rates a number of gram-positive bacteria. With the exception of Rhodospirillum rubrum, Spirillum itersonii , and S. serpens , the gram-negative bacteria tested were not attacked.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference20 articles.

1. Untersuchungen an Myxococcus xanthus. I. Bildungsbedingungen, Isolierung und Eigenschaften eines baketeriolytischen Enzymsystems;BENDER H.;Arch. Mikrobiol.,1962

2. Taxonomic position of Arthrobacter;CUMMINS C. S.;Nature,1959

3. Requirements for vegetative growth of Myxococcus xanthus;DWORKIN M.;J. Bacteriol.,1962

4. Nutritional control of morphogenesis in Arthrobacter crystallopoietes;ENSIGN J. C.;J. Bacteriol.,1964

5. ENSIGN J. C. AND R. S. WOLFE. 1964b. A new cell-wall Iytic enzyme. Bacteriol. Proc. p. 33.

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