Purification of a Salt-Requiring Enzyme from an Obligately Halophilic Bacterium

Abstract

Holmes, P. K. (University of Illinois, Urbana), and H. O. Halvorson . Purification of a salt-requiring enzyme from an obligately halophilic bacterium. J. Bacteriol. 90: 312–315. 1965.—The discovery that some halophilic enzymes may be reactivated from the salt-free and inactive state has facilitated the purification of nicotinamide adenine dinucleotide-linked malic acid dehydrogenase from crude extracts of Halobacterium salinarium . In the absence of large amounts of salt, the enzyme was totally inactive; yet, in this salt-free state, it could be purified by conventional techniques. The highly purified halophilic enzyme was shown to require a high concentration of salt for activity.