Ultrastructure of a Mycoplasma (Negroni) Isolated from Human Leukemia

Author:

Hummeler Klaus1,Tomassini Natale1,Hayflick Leonard1

Affiliation:

1. Virus Laboratories at the Children's Hospital of Philadelphia, School of Medicine, University of Pennsylvania, and The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania

Abstract

Hummeler, Klaus (The Children's Hospital of Philadelphia, Philadelphia, Pa.), Natale Tomassini, and Leonard Hayflick . Ultrastructure of a mycoplasma (Negroni) isolated from human leukemia. J. Bacteriol. 90: 517–523. 1965.—A strain of mycoplasma isolated in tissue cultures from human leukemic bone marrow was cultivated in cell-free media for 23 passages. Concentrates of broth cultures were investigated under the electron microscope in thin sections and negative contrast. The ultrastructure of the mature mycoplasma cells and their elementary bodies is described. It was found that the elementary bodies, particularly in the negative contrast preparations, bear a close resemblance to viruslike particles seen in tissues and plasmas of leukemic patients.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference9 articles.

1. Virus-like particles in blood of two acute leukemic patients;ALMEIDA J. D.;Science,1963

2. BEARD J. W. 1959. Virus tumors in cancer research. Perspectives Virol. Symp. New York p. 197-230.

3. On the occurrence of virus-like bodies in human leukemia;BRAUNSTEINER H., K.;Blood,1960

4. Virus-like particles in human leukemic plasma;BURGER C. L.;Proc. Soc. Exptl. Biol. Med.,1964

5. Studies on submicroscopic structures of leukemias of known or suspected viral etiology: a review;DMOCHOWSKI L.;Blood,1958

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