Spread of Integron-Associated VIM-Type Metallo-β-Lactamase Genes among Imipenem-Nonsusceptible Pseudomonas aeruginosa Strains in Greek Hospitals-Reference-Cited by-同舟云学术

Spread of Integron-Associated VIM-Type Metallo-β-Lactamase Genes among Imipenem-Nonsusceptible Pseudomonas aeruginosa Strains in Greek Hospitals

Published:2003-02 Issue:2 Volume:41 Page:822-825
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Giakkoupi P.¹²,Petrikkos G.²,Tzouvelekis L. S.³,Tsonas S.¹,Legakis N. J.³,Vatopoulos A. C.¹

Affiliation:

1. Department of Hygiene & Epidemiology

2. Infectious Diseases and Antimicrobial Chemotherapy Research Laboratory “G.K. Daikos,”

3. Department of Microbiology, Medical School, Athens University, Greece

Abstract

ABSTRACT Fifty-eight imipenem-nonsusceptible (MIC ≥ 8 μg/ml) Pseudomonas aeruginosa strains isolated during May 2001 in 15 Greek hospitals were studied. Thirty-six isolates derived from nine hospitals carried VIM-type metallo-β-lactamase genes, as found by PCR. In 34 isolates, bla VIM was associated with class 1 integrons of various sizes. DNA sequencing indicated the presence of bla VIM-2 gene cassettes in a variety of integron structures. Random amplified polymorphic DNA typing suggested diversity of the bla VIM -positive strains. Synergy between 2-mercaptoacetic acid and imipenem indicated carbapenemase activity in 26 bla VIM -positive strains.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.2.822-825.2003

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