Rapid Identification of the Genus Fonsecaea by PCR with Specific Oligonucleotide Primers-Reference-Cited by-同舟云学术

Rapid Identification of the Genus Fonsecaea by PCR with Specific Oligonucleotide Primers

Published:2003-02 Issue:2 Volume:41 Page:873-876
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Abliz Paride¹,Fukushima Kazutaka¹,Takizawa Kayoko¹,Nieda Norikazu¹,Miyaji Makoto¹,Nishimura Kazuko¹

Affiliation:

1. Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan

Abstract

ABSTRACT An oligonucleotide primer set based on internal transcribed spacer regions of ribosomal DNA for PCR which gives the amplicon for only the DNA from Fonsecaea species was designed. This set yielded an amplicon with 333 bp for all strains of Fonsecaea pedrosoi and Fonsecaea compacta examined but no amplicons for related dematiaceous fungi and pathogenic yeasts. PCR using this primer set was considered to be a useful method for the rapid identification of the genus Fonsecaea .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.2.873-876.2003

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