Plasmid-Based System for High-Level Gene Expression and Antisense Gene Knockdown in Bartonella henselae-Reference-Cited by-同舟云学术

Plasmid-Based System for High-Level Gene Expression and Antisense Gene Knockdown in Bartonella henselae

Published:2009-08-15 Issue:16 Volume:75 Page:5434-5436
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Gillaspie Devin¹,Perkins Izabella¹,Larsen Kellie¹,McCord Amy¹,Pangonis Scott¹,Sweger Debra¹,Seleem Mohamed N.²,Sriranganathan Nammalwar³,Anderson Burt E.¹

Affiliation:

1. Department of Molecular Medicine, College of Medicine, University of South Florida, Tampa, Florida 33612

2. Institute for Critical Technology and Applied Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

3. Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

Abstract

ABSTRACT Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae . The vectors were used to express a β-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR , a putative transcription response regulator of B. henselae , this antisense RNA gene knockdown strategy reduced bacterial invasion of human endothelial cells by over 60%.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00949-09

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