Transduction of Well-Differentiated Airway Epithelium by Recombinant Adeno-Associated Virus Is Limited by Vector Entry-Reference-Cited by-同舟云学术

Transduction of Well-Differentiated Airway Epithelium by Recombinant Adeno-Associated Virus Is Limited by Vector Entry

Published:1999-07 Issue:7 Volume:73 Page:6085-6088
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Bals Robert¹,Xiao Weidong¹,Sang Nianli¹,Weiner Daniel J.¹²,Meegalla Rupalie L.¹,Wilson James M.¹

Affiliation:

1. Departments of Medicine and Molecular and Cellular Engineering, Institute for Human Gene Therapy, and The Wistar Institute,1 and

2. Division of Pulmonary Medicine, The Children’s Hospital of Philadelphia,2 Philadelphia, Pennsylvania 19104

Abstract

ABSTRACT The limitations of adeno-associated virus (AAV)-mediated vectors for lung-directed gene transfer were investigated by using differentiated human respiratory epithelium in air-liquid interface cultures. Transduction efficiency was high in undifferentiated cells and was enhanced in well-differentiated cells after basolateral application of the vector or after apical application following disruption of tight junctions or pretreatment of the cultures with glycosidases. These results indicate that transduction of airway epithelia by AAV vectors is limited by entry and reinforce the importance of a physical barrier on the airway surface.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.73.7.6085-6088.1999

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