Author:
Kanaly R,Bartha R,Fogel S,Findlay M
Abstract
To investigate the possible cometabolic biodegradation of benzo[a]pyrene (BaP), crude oil spiked with [7-(sup14)C]BaP and unlabeled BaP was added to soil with no known pollution history, to give 34 g of oil and 67 mg of BaP/kg of dry soil. The oil-soil mixture was amended with mineral nutrients and incubated in an airtight container with continuous forced aeration. Total CO(inf2) and (sup14)CO(inf2) in the off-gas were trapped and quantified. Soil samples were Soxhlet extracted with dichloromethane at seven time points during the 150-day incubation period, and the extracted soil was subjected to further fractionation in order to recover reversibly and irreversibly bound radiocarbon. Radiocarbon recovery was 100% (plusmn) 3% for each time point. During the first 50 days of incubation, no (sup14)CO(inf2) was evolved, but over the next 100 days, 50% of the BaP radiocarbon was evolved as (sup14)CO(inf2). At 150 days, only 5% of the intact BaP and 23% of the crude oil remained. Of the remaining radiolabel, 20% was found in solvent-extractable metabolites and 25% was incorporated into soil organic matter. Only 1/10 of this could be solubilized by chemical hydrolysis. An abiotic control experiment exhibited binding of only 2% of the BaP, indicating the microbial nature of the BaP transformations. We report that in soil containing suitable cosubstrates, BaP can be completely degraded.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference27 articles.
1. Atlas R. M. and R. Bartha. 1993. Microbial ecology: fundamentals and applications 3rd ed. p. 346-400. Benjamin Cummings Redwood City Calif.
2. Decontamination of soil through enhanced formation of bound residues;Berry D. F.;Environ. Sci. Technol.,1985
3. Bortiatynski J. M. P. G. Hatcher R. D. Minard J. Dec and J.-M. Bollag. 1994. Enzyme-catalyzed binding of 13 C-labeled 2 4-dichlorophenol to humic acid using high resolution 13 C NMR p. 1091-1098. In N. Senesi and T. M. Miano (ed.) Humic substances in the global environment and implications on human health. Elsevier Science B.V. Amsterdam The Netherlands.
4. Biodegradation of polycyclic aromatic hydrocarbons;Cerniglia C. E.;Biodegradation,1993
5. Fungal oxidation of benzo[a]pyrene: formation of (~)-trans-7,8-dihydroxy-7,8-dihydrobenzo[a] pyrene by Cunninghamella elegans;Cerniglia C. E.;Biochem. Biophys. Res. Commun.,1980
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