Isolation of a Sindbis virus variant by passage on mouse plasmacytoma cells

Author:

Symington J,Schlesinger M J

Abstract

A variant of Sindbis virus has been isolated by growing a stock of virus, previously passaged on chicken embryo cells, in mouse plasmacytoma (MOPC 315) cells in suspension culture. An indirect immunofluorescence test and infective center assay showed that only a small fraction of cells could be infected by the stock wild-type virus, but that the population of virus accumulating after a few passages on the mouse cells had host-range properties distinct from the stock virus. The mouse-passaged virus retained its virulence for the original host and back-passaging of this virus on chicken cells did not change its newly acquired properties. Thus, this variant appears to be a genetically distinct form of Sindbis that adsorbs to and grows much better than the stock virus on several types of mouse cells including cultures of mouse macrophages.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference22 articles.

1. Peptide composition of Sindbis virus variants;Bose H. R.;Arch. Gesamte Virusforsch.,1970

2. Purification of two plaque variants of Sindbis virus;Bose H. R.;Arch. Gesamte Virusforsch.,1970

3. Some properties of large and small plaques of Sindbis virus variants innoculated into baby mice;Bres P.;Ann. Inst. Pasteur Paris,1965

4. Comparison of membrane protein glycopeptide of Sindbis virus and vesicular stomatitis;Burge B. W.;J. Virol.,1970

5. Fenner F. and J. Cairns. 1959. Variation in virulence in relation to adaptation to new hosts p. 225-249. In F. M. Burnet and W. M. Stanley (ed.) The viruses vol. 3. Academic Press Inc. New York.

Cited by 17 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3