Author:
McLean B,Sonza S,Holmes I H
Abstract
A solid-phase, enzyme-linked immunospecific assay for measurement of different immunoglobulin classes of human rotavirus antibodies is described. The antigen, which was adsorbed directly to polyvinyl microtiter plates, consisted of a clarified cell culture stock of the simian rotavirus SA 11. The assay was sensitive and reproducible and could readily be calibrated to determine concentrations of each class of antibody. The assay was applied to measurements of rotavirus antibodies in serum, colostrum, milk, and fecal samples. It particularly facilitates investigations of the role of immunoglobulin A antibodies in immunity to rotavirus infections.
Publisher
American Society for Microbiology
Reference25 articles.
1. The enzyme-linked immunosorbent assay (ELISA);Bidwell D. E.;Bull. W. H. O.,1976
2. Immunization of infants and young children against rotaviral gastroenteritis-prospects and problems;Chanock R. M.;J. Am. Vet. Med. Assoc.,1978
3. Persistence of antibodies to rotavirus in human milk;Cukor G.;J. Clin. Microbiol.,1979
4. Davidson G. P. R. F. Bishop R. R. W. Townley I. H. Holmes and B. J. Ruck. 1975. Importance of a new virus in acute sporadic enteritis in children. Lancet i:242-246.
5. The rotaviruses;Flewett T. H.;Arch. Virol.,1978
Cited by
37 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献