Affiliation:
1. Molecular Biology Section, Veterinary Research Institute, Onderstepoort, South Africa
Abstract
An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 × 10
6
to 2.8 × 10
6
daltons, with a total molecular weight estimate of about 1.5 × 10
7
for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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