Endogenous Metabolism of Azotobacter agilis

Author:

Sobek J. M.1,Charba J. F.1,Foust W. N.1

Affiliation:

1. Department of Microbiology, University of Southwestern Louisiana, Lafayette, Louisiana

Abstract

Sobek , J. M. (University of Southwestern Louisiana, Lafayette), J. F. Charba, and W. N. Foust . Endogenous metabolism of Azotobacter agilis . J. Bacteriol. 92: 687–695. 1966—Ribonucleic acid, deoxyribonucleic acid, cellular carbohydrate, and the cold trichloroacetic acid and acidic alcohol fractions of the cell do not appear to function as endogenous reserves for Azotobacter agilis . The immediate endogenous reserve of cells grown on glucose, acetate, or succinate was poly-β-hydroxybutyric acid (PHB). Viability of the cells during starvation was dependent upon the initial levels of PHB and the growth substrate. Cells with high initial PHB levels survived longer than cells with lower levels. Cells from succinate-grown cultures had lower PHB levels than cells from glucose-grown cultures, but were capable of maintaining their viability longer. Cellular protein may also serve as a secondary endogenous reserve substrate for this organism.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference21 articles.

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5. HARROW B. E. BOREK A. MAZUR G. C. H. STONE AND H. WAGREICH. 1960. Laboratory manual of biochemistry 5th ed. p. 34-35. W. B. Saunders Co. Philadelphia.

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