Author:
Tennent J M,Lyon B R,Gillespie M T,May J W,Skurray R A
Abstract
The Staphylococcus aureus plasmid pSK1 carries Tn4001, a 4.7-kilobase (kb) transposon which specifies resistance to gentamicin, tobramycin, and kanamycin. In addition, pSK1 mediates resistance to trimethoprim and linked resistance to ethidium bromide (Ebr) and to quaternary ammonium compounds (Qar). Restriction endonuclease analysis of pSK1 and a deleted derivative of pSK1 revealed that the gene(s) responsible for Ebr Qar lies within a 5.2-kb HindIII fragment. This fragment has been cloned into the Escherichia coli plasmid vector pBR322, and transformants of an E. coli K-12 strain exhibited Ebr Qar. Subcloning of the 5.2-kb insert, combined with data from electron microscopic analysis of deleted derivatives of pSK1, located the Ebr Qar determinant(s) on a 2.3-kb segment of pSK1 DNA.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference17 articles.
1. Self-transmissible plasmids in staphylococci that encode resistance to aminoglycosides;Archer G. L.;Antimicrob. Agents Chemother.,1983
2. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system;Bolivar F.;Gene,1977
3. Daniels D. L. J. L. Schroeder W. Szybalski F. Sanger and F. R. Blattner. 1983. A molecular map of coliphage lambda p. 469-517. In R. W. Hendrix J. W. Roberts F. W. Stahl and R. A. Weisberg (ed.) Lambda II. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
4. Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids;Davis R. E.;Methods Enzymol.,1971
5. In vitro packaging of X and cosmid DNA;Hohn B.;Methods Enzymol.,1979
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