Author:
Farrah S R,Scheuerman P R,Bitton G
Abstract
Enteroviruses added to sludge and indigenous viruses present in sludge were recovered by treating the sludge flocs with a 4 M urea solution buffered at pH 9 with 0.5 M lysine. Eluted viruses were absorbed to aluminum hydroxide flocs and collected by centrifugation. The flocs were solubilized with 0.1 M ethylenediaminetetraacetic acid-3% beef extract at pH 9. After dialysis to remove the ethylenediaminetraacetic acid, viruses were further concentrated by organic flocculation. Approximately 40% of poliovirus and coxsackievirus B-3 added to 500 to 1,000 ml of sludge could be recovered in final sample volumes of less than 10 ml. Polioviruses, echoviruses, and coxsackieviruses were recovered from different samples of wastewater sludge.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference15 articles.
1. Effect of a secondary treated effluent on the movement of virus through a cypress dome soil;Bitton G.;J. Environ. Qual.,1975
2. Removal of enteric viruses from sewage by activated sludge treatment;Clarke N. A.;Am. J. Pub. Hlth.,1961
3. Low molecular weight substitutes for beef extract as eluents for polioviruses absorbed to membrane filters;Farrah S. R.;Can. J. Microbiol.,1979
4. Survival of enteroviruses and coliform bacteria in a sludge lagoon;Farrah S. R.;Appl. Environ. Microbiol.,1981
5. Concentration of enteroviruses from estuarine water;Farrah S. R.;Appl. Environ. Microbiol.,1977
Cited by
17 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献