Improved enrichment broth for cultivation of fastidious organisms

Author:

Cartwright C P1,Stock F1,Gill V J1

Affiliation:

1. Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892.

Abstract

An enrichment broth developed in our laboratory, fastidious broth (FB), was compared with two commercially available broth media, supplemented thioglycolate broth and enriched eugonic broth. FB supported the growth of a number of organisms that were not cultivatable in either of the other two media, including Corynebacterium jeikeium, Haemophilus influenzae, Neisseria gonorrhoeae, and Streptococcus pneumoniae. In addition, for several organisms that were able to grow in all three broths, including Neisseria meningitidis, Nocardia asteroides, and Actinomyces spp., both the time of incubation and the starting inoculum necessary to enable detection of growth were decreased significantly by using FB.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference3 articles.

1. Laboratory diagnosis of bacterial meningitis;Gray L. D.;Clin. Microbiol. Rev.,1992

2. Isenberg H. D. E. J. Baron R. F. D'Amato R. C. Johnson P. R. Murray F. G. Rodgers and A. von Graevenitz. 1991. Recommendations for the isolation of bacteria from clinical specimens p. 216-221. In A. Balows W. J. Hausler Jr. K. L. Herrmann H. D. Isenberg and H. J. Shadomy (ed.) Manual of clinical microbiology 5th ed. American Society for Microbiology Washington D.C.

3. Sutter V. L. D. M. Citron M. A. C. Edelstein and S. M. Finegold. 1986. Wadsworth anaerobic bacteriology manual 4th ed. p. 23-70. Star Publishing Co. Belmont Calif.

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