Evaluation of Alloreactivity in Responder-Stimulator Pairs by Determination of Gamma Interferon-Producing Cells and Cytotoxic-T-Lymphocyte Precursor Frequencies-Reference-Cited by-同舟云学术

Evaluation of Alloreactivity in Responder-Stimulator Pairs by Determination of Gamma Interferon-Producing Cells and Cytotoxic-T-Lymphocyte Precursor Frequencies

Published:2007-04 Issue:4 Volume:14 Page:481-483
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Sizzano Federico¹,Dametto Ennia¹,Amoroso Antonio¹

Affiliation:

1. Transplantation Immunology, San Giovanni Battista Hospital, Turin, and Department of Genetics, Biology and Biochemistry, University of Turin, Turin, Italy

Abstract

ABSTRACT We used the enzyme-linked immunospot (ELISPOT) assay and the cytotoxic-T-lymphocyte precursor frequency assay to evaluate alloreactivity in responder-stimulator pairs. High frequencies of responder cells among cells from HLA-mismatched pairs and low frequencies among cells from pairs of siblings with identical HLA types were detected by both assays. The ELISPOT assay thus illustrated the helper and cytotoxic-T-cell response to allogeneic HLA antigens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00402-06

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