Assessment of enzyme immunoassay versus radioimmunoassay for detection of Legionella pneumophila serogroup 1 antigen in frozen urine specimens-Reference-Cited by-同舟云学术

Assessment of enzyme immunoassay versus radioimmunoassay for detection of Legionella pneumophila serogroup 1 antigen in frozen urine specimens

Published:1996-10 Issue:10 Volume:34 Page:2628-2629
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Chang F Y¹,Stout J E¹,Yu V L¹

Affiliation:

1. Special Pathogens Laboratory, Veterans Affairs Medical Center, Pittsburgh, Pennsylvania, USA.

Abstract

An enzyme immunoassay (EIA) was compared with a radioimmunoassay (RIA) for detection of Legionella pneumophila serogroup 1 antigen in 37 frozen urine samples. Among 18 samples positive by the RIA at the time of collection, 50% (9 of 18) of stored specimens retained positivity when retested by the RIA whereas 78% (14 of 18) of stored specimens retained positivity when retested by the EIA (P < 0.02). A false-negative result occurred after storage for 5 months or less if the original RIA result showed weak positivity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.34.10.2628-2629.1996

Reference9 articles.

1. Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans;Aguero-Rosenfeld M. E.;J. Clin. Microbiol.,1988

2. Comparison of Binax Legionella Urinary Antigen EIA kit with Binax RIA Urinary Antigen kit for detection of Legionella pneumophila serogroup 1 antigen;Hackman B. A.;J. Clin. Microbiol.,1996

3. Kohler R. B. 1986. Legionella pneumophila p. 135-153. In R. B. Kohler (ed.) Antigen detection to diagnose bacterial infections vol. 2. Applications. CRC Press Inc. Boca Raton Fla.

4. Rapid radioimmunoassay diagnosis of Legionnaires' disease: detection and partial characterization of urinary antigen;Kohler R. B.;Ann. Intern. Med.,1981

5. Muro V. and R. C. Tilton. 1995. Comparison of EIA and RIA for the detection of Legionella pneumophila I urinary antigen abstr. C-168 p. 30. In Abstracts of the 95th General Meeting of the American Society for Microbiology 1995. American Society for Microbiology Washington D.C.

Cited by 16 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Comparison of the ImmuView and the BinaxNOW antigen tests in detection of Streptococcus pneumoniae and Legionella pneumophila in urine;European Journal of Clinical Microbiology & Infectious Diseases;2017-06-06

2. Evaluation of the bioNexia Legionella Test, Including Impact of Incubation Time Extension, for Detection of Legionella pneumophila Serogroup 1 Antigen in Urine;Journal of Clinical Microbiology;2017-06

3. Development and pre-validation of a testosterone enzyme immunoassay (EIA) for predicting the sex ratio of immature loggerhead sea turtles (Caretta caretta) recovered along the western coast of the central Adriatic Sea;Marine Biology;2013-09-26

4. Immunology;Clinical Microbiology Procedures Handbook;2010-08

5. Destruction of Isolates from the Pittsburgh Veterans Affairs Laboratory;Clinical Infectious Diseases;2008-04