Mutations in the 3'-PPT Lead to HIV-1 Replication without Integration-Reference-Cited by-同舟云学术

Mutations in the 3'-PPT Lead to HIV-1 Replication without Integration

Published:2022-07-27 Issue:14 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Richetta Clémence¹,Subra Frédéric¹,Malet Isabelle²,Leh Hervé¹,Charpentier Charlotte³,Corona Angela⁴,Collin Gilles³,Descamps Diane³,Deprez Eric¹,Parissi Vincent⁵^ORCID,Calvez Vincent²,Tramontano Enzo⁴^ORCID,Marcelin Anne-Geneviève²,Delelis Olivier¹^ORCID

Affiliation:

1. LBPA, ENS Paris-Saclay, CNRS UMR8113, IDA FR3242, Université Paris-Saclay, Cachan, France

2. Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP) AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire de virologie, Paris, France

3. INSERM, IAME, UMR 1137, Paris, France; Université Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France; AP-HP, Hôpital Bichat, Laboratoire de Virologie, Paris, France

4. Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Monserrato, Italy

5. Fundamental Microbiology and Pathogenicity Laboratory, UMR 5234 CNRS-University of Bordeaux, SFR TransBioMed, Bordeaux Cedex, France

Abstract

Our work highlights the role of HIV-1 unintegrated viral DNA in viral replication. A virus, resistant to strand-transfer inhibitors, has been selected in vitro . This virus highlights a mutation in the 3'PPT region and not in the integrase gene. This mutation modifies the reverse transcription step leading to the accumulation of 1-LTR circles and not the linear DNA. This accumulation of 1-LTR circles leads to viral replication without integration of the viral genome.

Funder

Agence Nationale de Recherches sur le Sida et les Hépatites Virales

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.00676-22

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