Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay-Reference-Cited by-同舟云学术

Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay

Published:1996-06 Issue:6 Volume:34 Page:1465-1467
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Klespies S L¹,Cebula D E¹,Kelley C L¹,Galehouse D¹,Maurer C C¹

Affiliation:

1. Department of Pathology and Laboratory Medicine, Children's Hospital Medical Center of Akron, Ohio 44308, USA.

Abstract

Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interval. The data suggest that a spin-amplified shell vial indirect immunofluorescence assay may be useful for the detection of enterovirus from clinical specimens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.34.6.1465-1467.1996

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