The hmsHFRS Operon of Xenorhabdus nematophila Is Required for Biofilm Attachment to Caenorhabditis elegans

Abstract

ABSTRACT The bacterium Xenorhabdus nematophila is an insect pathogen and an obligate symbiont of the nematode Steinernema carpocapsae. X. nematophila makes a biofilm that adheres to the head of the model nematode Caenorhabditis elegans , a capability X. nematophila shares with the biofilms made by Yersinia pestis and Yersinia pseudotuberculosis . As in Yersinia spp., the X. nematophila biofilm requires a 4-gene operon, hmsHFRS . Also like its Yersinia counterparts, the X. nematophila biofilm is bound by the lectin wheat germ agglutinin, suggesting that β-linked N -acetyl- d -glucosamine or N -acetylneuraminic acid is a component of the extracellular matrix. C. elegans mutants with aberrant surfaces that do not permit Yersinia biofilm attachment also are resistant to X. nematophila biofilms. An X. nematophila hmsH mutant that failed to make biofilms on C. elegans had no detectable defect in symbiotic association with S. carpocapsae , nor was virulence reduced against the insect Manduca sexta .