Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography-Reference-Cited by-同舟云学术

Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography

Published:1982-08 Issue:2 Volume:16 Page:355-360
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Guerrant G O,Lambert M A,Moss C W

Abstract

A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fractions from the high-performance liquid chromatography column.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.16.2.355-360.1982

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