Affiliation:
1. Eaton Laboratories, The Norwich Pharmacal Company, Norwich, New York 13815
Abstract
A lipase which hydrolyzes triglycerides (tricaprylin and trilaurin) and naphthyl laurate was obtained from the broth of
Corynebacterium acnes
cultures by ammonium sulfate fractionation. Ca
2+
and sodium taurocholate stimulated activity of the enzyme. Ethylenediaminetetraacetic acid (EDTA) did not inhibit activity of the Ca
2+
-activated enzyme, but lipolytic activity was inhibited by EDTA in the absence of Ca
2+
. Tetracycline (10
−4
m
) produced a slight inhibition of the lipase activity with 5 × 10
−5
m
or less showing no effect on the lipase activity. However, complete inhibition by tetracycline at 10
−4
m
was observed for Ca
2+
-activated enzyme. Tetracycline inhibition of the
C. acnes
lipase could be demonstrated at concentrations as low as 10
−6
m
.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference16 articles.
1. Untersuchungen uber die wirkung der tetracycline bei der acne vulgaris;Faget H.;Hautarzt,1968
2. Effect of tetracycline on the composition of sebum in acne vulgaris;Freinkel R.;N. Engl. J. Med.,1965
3. Metal chelate compounds of tetracycline derivatives;Ishidate M.;I. Aureomycin. Chem. Pharm. Bull. (Tokyo),1955
4. Triglyceride hydrolysis by Corynebacterium acnes in vitro;Kellum R. E.;J. Invest. Dermiatol.,1969
5. Acne vulgaris studies in pathogenesis: triglyceride hydrolysis by Corynebacterium acnes in vitro;Kellum R. E.;Arch. Dermatol.,1970
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献