Affiliation:
1. Department of Microbiology and Department of Organic Chemistry, 2 University of Cape Town, Rondebosch 7700, South Africa
Abstract
Synthesis of granulose was investigated in 15 solvent-producing
Clostridium
strains. Only one of the strains did not produce granulose. The structure of granulose in
Clostridium acetobutylicum
P262 consisted of a high-molecular-weight polyglucan containing only (1→4) linked
d
-glucopyranose units. Biosynthesis of granulose in
C. acetobutylicum
P262 was dependent on ADPglucose pyrophosphorylase, and granulose synthase and mutants defective in granulose accumulation lacked either one or both enzyme activities. Granulose-positive revertants exhibited both enzyme activities. ADPglucose pyrophosphorylase and granulose synthase were not subject to allosteric control by metabolites. Granulose accumulation and the biosynthetic enzyme activities were initiated immediately before the pH breakpoint and were detected in cells only at the end of the exponential growth phase. Granulose accumulation did not occur under conditions of nitrogen limitation, excess carbon, or excess energy.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
36 articles.
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