Use of 4-Sulfophenyl Isothiocyanate Labeling and Mass Spectrometry To Determine the Site of Action of the Streptococcolytic Peptidoglycan Hydrolase Zoocin A-Reference-Cited by-同舟云学术

Use of 4-Sulfophenyl Isothiocyanate Labeling and Mass Spectrometry To Determine the Site of Action of the Streptococcolytic Peptidoglycan Hydrolase Zoocin A

Published:2009-01 Issue:1 Volume:75 Page:72-77
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Gargis Shaw R.¹,Heath Harry E.¹,Heath Lucie S.¹,LeBlanc Paul A.¹,Simmonds Robin S.²,Abbott Brian D.³,Timkovich Russell³,Sloan Gary L.¹

Affiliation:

1. Department of Biological Sciences, The University of Alabama, Tuscaloosa, Alabama

2. Department of Microbiology, University of Otago, Dunedin, New Zealand

3. Department of Chemistry, The University of Alabama, Tuscaloosa, Alabama

Abstract

ABSTRACT Zoocin A is a streptococcolytic peptidoglycan hydrolase with an unknown site of action that is produced by Streptococcus equi subsp. zooepidemicus 4881. Zoocin A has now been determined to be a d -alanyl- l -alanine endopeptidase by digesting susceptible peptidoglycan with a combination of mutanolysin and zoocin A, separating the resulting muropeptides by reverse-phase high-pressure liquid chromatography, and analyzing them by mass spectrometry (MS) in both the positive- and negative-ion modes to determine their compositions. In order to distinguish among possible structures for these muropeptides, they were N-terminally labeled with 4-sulfophenyl isothiocyanate (SPITC) and analyzed by tandem MS in the negative-ion mode. This novel application of SPITC labeling and MS/MS analysis can be used to analyze the structure of peptidoglycans and to determine the sites of action of other peptidoglycan hydrolases.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01647-08

Reference23 articles.

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