Affiliation:
1. College of Life Sciences, Wuhan University, Wuhan, China
2. Hubei Provincial Hospital of TCM, Wuhan, China
Abstract
ABSTRACT
Stenotrophomonas maltophilia
is an important global opportunistic pathogen for which limited therapeutics are available because of the emergence of multidrug-resistant strains. A novel bacteriocin, maltocin P28, which is produced by
S. maltophilia
strain P28, may be the first identified phage tail-like bacteriocin from
S. maltophilia
. Maltocin P28 resembles a contractile but nonflexible phage tail structure based on electron microscopy, and it is sensitive to trypsin, proteinase K, and heat. SDS-PAGE analysis of maltocin P28 revealed two major protein bands of approximately 43 and 20 kDa. The N-terminal amino acid residues of these two major subunits were sequenced, and the maltocin P28 gene cluster was located on the
S. maltophilia
P28 chromosome. Our sequence analysis results indicate that this maltocin gene cluster consists of 23 open reading frames (ORFs), and that its gene organization is similar to that of the P2 phage genome and R2 pyocin gene cluster. ORF17 and ORF18 encode the two major structural proteins, which correspond to gpFI (tail sheath) and gpFII (tail tube) of P2 phage, respectively. We found that maltocin P28 had bactericidal activity against 38 of 81 tested
S. maltophilia
strains. Therefore, maltocin P28 is a promising therapeutic substitute for antibiotics for
S. maltophilia
infections.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
36 articles.
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