Novel Glycoside Hydrolases Identified by Screening a Chinese Holstein Dairy Cow Rumen-Derived Metagenome Library

Author:

Zhao Shengguo1,Wang Jiaqi1,Bu Dengpan1,Liu Kailang1,Zhu Yaxin2,Dong Zhiyang2,Yu Zhongtang3

Affiliation:

1. State Key Laboratory of Animal Nutrition, the Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China

2. State Key Laboratory of Microbial Resources, the Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China

3. Department of Animal Sciences, Ohio State University, Columbus, Ohio 43210

Abstract

ABSTRACT One clone encoding glycoside hydrolases was identified through functional screening of a rumen bacterial artificial chromosome (BAC) library. Of the 68 open reading frames (ORFs) predicted, one ORF encodes a novel endo-β-1,4-xylanase with two catalytic domains of family GH43 and two cellulose-binding modules (CBMs) of family IV. Partial characterization showed that this endo-xylanase has a greater specific activity than a number of other xylanases over a wide temperature range at neutral pH and could be useful in some industrial applications.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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