Affiliation:
1. Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242
Abstract
ABSTRACT
The
Candida albicans
gene
EFG1
encodes a putative
trans
-acting factor. In strain WO-1, which undergoes the white-opaque transition,
EFG1
is transcribed as a 3.2-kb mRNA in white-phase cells and a less-abundant 2.2-kb mRNA in opaque-phase cells. cDNA sequencing and 5′ rapid amplification of cDNA ends analysis demonstrate that the major difference in molecular mass of the two transcripts is due to different transcription start sites.
EFG1
null mutants form opaque-phase colonies and express the opaque-phase cell phenotype at 25°C. When shifted from 25 to 42°C, mutant opaque-phase cells undergo phenotypic commitment to the white phase, which includes deactivation of the opaque-phase-specific gene
OP4
and activation of the white-phase-specific gene
WH11
, as do wild-type opaque-phase cells. After the commitment event,
EFG1
null mutant cells form daughter cells which have the smooth (pimpleless) surface of white-phase cells but the elongate morphology of opaque-phase cells. Taken together, these results demonstrate that
EFG1
expression is not essential for the switch event per se, but is essential for a subset of phenotypic characteristics necessary for the full expression of the phenotype of white-phase cells. These results demonstrate that
EFG1
is not the site of the switch event, but is, rather, downstream of the switch event.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
106 articles.
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