A Two Amino Acid Duplication, L167E168, in the Ω-Loop Drastically Decreases Carbapenemase Activity of KPC-53, a Natural Class A β-Lactamase-Reference-Cited by-同舟云学术

A Two Amino Acid Duplication, L167E168, in the Ω-Loop Drastically Decreases Carbapenemase Activity of KPC-53, a Natural Class A β-Lactamase

Published:2022-06-21 Issue:6 Volume:66 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Piccirilli Alessandra¹,Cherubini Sabrina¹,Celenza Giuseppe¹,Rossolini Gian Maria²³^ORCID,Brisdelli Fabrizia¹,Segatore Bernardetta¹,Principe Luigi⁴,Luzzaro Francesco⁴,Andriani Lilia⁵,Amicosante Gianfranco¹,Perilli Mariagrazia¹^ORCID

Affiliation:

1. Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy

2. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

3. Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence Italy

4. Clinical Microbiology and Virology Unit, A. Manzoni Hospital, Lecco, Italy

5. Clinical Pathology and Microbiology Unit, Moriggia Pelascini General Hospital, Gravedona, Italy

Abstract

KPC-53 enzyme is a natural KPC variant which showed a duplication of L167E168 residues in the Ω-loop structure. The bla KPC-53 gene was cloned both into pBC-SK and pET-24a vectors, and the recombinant plasmids were transferred by transformation in Escherichia coli competent cells to evaluate the antimicrobial susceptibility and to produce the enzyme.

Funder

Università degli Studi dell'Aquila

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/aac.02402-21

Reference31 articles.

1. Novel Carbapenem-Hydrolyzing β-Lactamase, KPC-1, from a Carbapenem-Resistant Strain of Klebsiella pneumoniae

2. IncX3 plasmid harboring a non-Tn 4401 genetic element (NTE KPC ) in a hospital-associated clone of KPC-2-producing Klebsiella pneumoniae ST340/CG258

Comparative analysis of KPC-2-encoding chimera plasmids with multi-replicon IncR:Inc_pA1763-KPC:IncN1 or IncFII_pHN7A8:Inc_pA1763-KPC: IncN1

4. Comparative genomic analyses of Inc pA1763‐KPC plasmids

5. Molecular characterization of carbapenem-resistant Klebsiella pneumoniae ST14 and ST512 causing bloodstream infections in ICU and surgery wards of a tertiary university hospital of Verona (northern Italy): co-production of KPC-3, OXA-48, and CTX-M-15 β-lactamases

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