Affiliation:
1. Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012
2. Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560064, India
Abstract
ABSTRACT
The two genes encoding DNA gyrase in
Mycobacterium tuberculosis
are present next to each other in the genome, with
gyrB
upstream of
gyrA
. We show that the primary transcript is dicistronic. However, in addition to the principal promoter, there are multiple weaker promoters that appear to fine-tune transcription. With these and other mycobacterial promoters, we propose consensus promoter sequences for two distinct sigma factors. In addition to this, the
gyr
genes in
M. tuberculosis
, as in other species, are subject to autoregulation, albeit with slower kinetics, probably reflecting the slower metabolism of the organism.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference29 articles.
1. Bachmann B. J. 1987. Linkage map of Escherichia coli K-12 edition 7 p. 807-877. In F. C. Neidhardt et al. (ed.) Escherichia coli and Salmonella typhimurium : cellular and molecular biology. American Society for Microbiology Washington D.C.
2. Bannantine, J. P., R. G. Barletta, C. O. Thoen, and R. E. Andrews, Jr. 1997. Identification of Mycobacterium paratuberculosis gene expression signals. Microbiology143:921-928.
3. A study of mycobacterial transcriptional apparatus: identification of novel features in promoter elements
4. Campbell, E. A., O. Muzzin, M. Chlenov, J. L. Sun, C. A. Olson, O. Weinman, M. L. Trester-Zedlitz, and S. A. Darst. 2002. Structure of the bacterial RNA polymerase promoter specificity sigma subunit. Mol. Cell9:527-539.
5. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
Cited by
62 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献