Development of a Novel Screening Method for the Isolation of “ Cronobacter ” spp. ( Enterobacter sakazakii )

Author:

Iversen Carol12,Druggan Patrick3,Schumacher Sandra1,Lehner Angelika1,Feer Claudia4,Gschwend Karl4,Joosten Han2,Stephan Roger1

Affiliation:

1. Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland

2. Quality and Safety Department, Nestlé Research Centre, Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland

3. Oxoid Ltd., Thermo Fisher Scientific, Basingstoke, Hampshire RG24 8PW, United Kingdom

4. QA and Food Safety Department, Hochdorf Nutritec AG, CH-6280 Hochdorf, Switzerland

Abstract

ABSTRACT A differential medium, “ Cronobacter ” screening broth, has been designed to complement agars based on hydrolysis of chromogenic α-glucopyranoside substrates. The broth was evaluated using 329 Enterobacteriaceae strains (229 target isolates), spiked/naturally contaminated samples, and a parallel comparison with current methods for raw materials, line/end products, and factory environment samples.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference22 articles.

1. Isolation and enumeration of Enterobacter sakazakii from dehydrated powdered infant formula. 2002

2. MRA Series 6. 2004

3. Second Risk Assessment Workshop 2006

4. Technical specification ISO/TS 22964. ISO/TS 22964:2006(E) and IDF/RM 210:2006(E) 2006

5. Gnanou Besse, N., A. Leclercq, V. Maladen, C. Tyburski, and B. Lombard. 2006. Evaluation of the International Organization for Standardization-International Dairy Federation (ISO-IDF) draft standard method for detection of Enterobacter sakazakii in powdered infant food formulas. J. AOAC Int.89:1309-1316.

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