Affiliation:
1. Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan
2. Center for Gene Research, Nagoya University, Nagoya, Japan
Abstract
ABSTRACT
The marine bacterium
Vibrio alginolyticus
has a single polar flagellum. Formation of that flagellum is regulated positively and negatively by FlhF and by FlhG, respectively. The Δ
flhF
mutant makes no flagellum, whereas the Δ
flhFG
double-deletion mutant usually lacks a flagellum. However, the Δ
flhFG
mutant occasionally reverts to become motile by forming peritrichous flagella. We have isolated a suppressor pseudorevertant from the Δ
flhFG
strain (Δ
flhFG
-sup). The suppressor strain forms peritrichous flagella in the majority of cells. We identified candidate suppressor mutations by comparing the genome sequence of the parental strain, VIO5, with the genome sequences of the suppressor strains. Two mutations were mapped to a gene, named
sflA
(
s
uppressor of Δ
fl
hFG
), at the VEA003730 locus of the
Vibrio
sp. strain EX25 genome. This gene is specific for
Vibrio
species and is predicted to encode a transmembrane protein with a DnaJ domain. When the wild-type gene was introduced into the suppressor strain, motility was impaired. Introducing a mutant version of the
sflA
gene into the Δ
flhFG
strain conferred the suppressor phenotype. Thus, we conclude that loss of the
sflA
gene is responsible for the suppressor phenotype and that the wild-type SflA protein plays a role in preventing polar-type flagella from forming on the lateral cell wall.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference50 articles.
1. The Epidemiology of Vibrio Infections in Florida, 1981-1993
2. ReillyGD ReillyCA SmithEG Baker-AustinC. 2011. Vibrio alginolyticus-associated wound infection acquired in British waters, Guernsey, July 2011. Euro Surveill. 16(42): pii=19994. http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19994.
3. Wound Infection Associated with Vibrio alginolyticus
4. Chronic Diarrhea Associated withVibrio alginolyticusin an Immunocompromised Patient
5. Prokaryotic motility structures
Cited by
31 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献