Affiliation:
1. Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
Abstract
ABSTRACT
PpLSU3, a mobile group I intron in the rRNA genes of
Physarum polycephalum
, also can home into yeast chromosomal ribosomal DNA (rDNA) (D. E. Muscarella and V. M. Vogt, Mol. Cell. Biol. 13:1023–1033, 1993). By integrating PpLSU3 into the rDNA copies of a yeast strain temperature sensitive for RNA polymerase I, we have shown that the I-
Ppo
I homing endonuclease encoded by PpLSU3 is expressed from an RNA polymerase I transcript. We have also developed a method to integrate mutant forms of PpLSU3 as well as the
Tetrahymena
intron TtLSU1 into rDNA, by expressing I-
Ppo
I in
trans
. Analysis of I-
Ppo
I expression levels in these mutants, along with subcellular fractionation of intron RNA, strongly suggests that the full-length excised intron RNA, but not RNAs that are further cleaved, serves as or gives rise to the mRNA.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
28 articles.
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