Herpes simplex virus type 1 ICP8: helix-destabilizing properties-Reference-Cited by-同舟云学术

Herpes simplex virus type 1 ICP8: helix-destabilizing properties

Published:1993-02 Issue:2 Volume:67 Page:711-715
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Boehmer P E¹,Lehman I R¹

Affiliation:

1. Department of Biochemistry, Beckman Center, Stanford University School of Medicine, California 94305-5307.

Abstract

The major single-stranded DNA-binding protein, ICP8, of herpes simplex virus type 1 (HSV-1) is one of seven virus-encoded polypeptides required for HSV-1 DNA replication. To investigate the role of ICP8 in viral DNA replication, we have examined the interaction of ICP8 with partial DNA duplexes and found that it can displace oligonucleotides annealed to single-stranded M13 DNA. In addition, ICP8 can melt small fragments of fully duplex DNA. Unlike a DNA helicase, ICP8-promoted strand displacement is ATP and Mg2+ independent and exhibits no directionality. It requires saturating amounts of ICP8 and is both efficient and highly cooperative. These properties make ICP8 suitable for a role in DNA replication in which ICP8 destabilizes duplex DNA during origin unwinding and replication fork movement.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.67.2.711-715.1993

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