Affiliation:
1. Centre National de la Recherche Scientifique Unité Associée 271, Institut Pasteur, Paris, France.
Abstract
The possibility of transfer of genetic information by conjugation from gram-positive to gram-negative bacteria was investigated with a pBR322-pAM beta 1 chimeric plasmid, designated pAT191. This shuttle vector, which possesses the tra functions of the streptococcal plasmid pAM beta 1, was conjugatively transferred from Enterococcus faecalis to Escherichia coli with an average frequency of 5 x 10(-9) per donor colony formed after mating.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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